SSR Screening and Primers in Brachypodium BAC End Sequences

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Index	Seq ID	Count	Primer_type	Orientation	Start	Len	Tm	GC%	Any compl	3' compl	Seq	Prod Size	Seq Length	Included Length	Pair any compl	Pair 3' compl	Motif	Motif Len	SSR	SSRLen
1	DB092D16_F1.ab1	1	SSR	FORWARD	85	21	55.88	42.86	5.00	1.00	CTCGACTGCACTTTAAGACAA	163	947	947	4.00	1.00	CTAG	4	ctagctagctag	12
2	DB092D16_F1.ab1	1	SSR	REVERSE	247	21	55.47	42.86	3.00	2.00	GCTGTTCACTGTTGAGGATTA
3	DB092D16_F1.ab1	1	SSR	FORWARD	509	20	54.92	50.00	3.00	2.00	GTCCACTCGTCAAGATTAGC	149	947	947	3.00	1.00	GCTA	4	gctagctagcta	12
4	DB092D16_F1.ab1	1	SSR	REVERSE	657	22	55.12	40.91	4.00	2.00	GTAGCTGTCCATCAATTAAGGT
5	DB086I03_R1.ab1	1	SSR	FORWARD	528	21	55.44	42.86	6.00	2.00	CCATTGTCTAGTGCTAATGGA	157	933	933	5.00	0.00	TGTG	4	tgtgtgtgtgtg	12
6	DB086I03_R1.ab1	1	SSR	REVERSE	684	21	54.70	42.86	6.00	2.00	GTTGAGCTAACAAGCTGAAAG
7	DB068O19_R1.ab1	1	SSR	FORWARD	15	21	55.41	42.86	4.00	0.00	CCGAGGGAGTAATAATAATGG	143	932	932	3.00	2.00	GCA	3	gcagcagcagcagca	15
8	DB068O19_R1.ab1	1	SSR	REVERSE	157	21	54.92	42.86	6.00	3.00	TGGAGAGGTCAGTTGATCTTA
9	DB096B10_R1.ab1	1	SSR	FORWARD	50	19	54.65	57.89	1.00	1.00	CACAGAGGGAGGAAGAGAC	129	928	928	4.00	1.00	GCC	3	gccgccgccgcc	12
10	DB096B10_R1.ab1	1	SSR	REVERSE	178	19	54.55	52.63	6.00	2.00	GGATCCTGGAGGCTATATG
11	DB096B10_R1.ab1	1	SSR	FORWARD	50	19	54.65	57.89	1.00	1.00	CACAGAGGGAGGAAGAGAC	129	928	928	4.00	1.00	CCG	3	ccgccgccgccgccgccg	18
12	DB096B10_R1.ab1	1	SSR	REVERSE	178	19	54.55	52.63	6.00	2.00	GGATCCTGGAGGCTATATG
13	DB090B06_F1.ab1	1	SSR	FORWARD	619	20	55.35	50.00	3.00	2.00	GTAGCAGCACCCTGTTGTAT	156	927	927	5.00	1.00	AGC	3	agcagcagcagc	12
14	DB090B06_F1.ab1	1	SSR	REVERSE	774	21	54.64	42.86	5.00	1.00	GTGCAGACAACTTCTTTCACT
15	DB081A05_R2.ab1	1	SSR	FORWARD	366	18	55.47	55.56	4.00	0.00	GGTTAGCACTGACGTGGA	231	925	925	3.00	2.00	ACAC	4	acacacacacacacacacac	20
16	DB081A05_R2.ab1	1	SSR	REVERSE	596	19	55.39	42.11	4.00	2.00	GAAAACAAAAGCGAAGCTC
17	DB081A05_R2.ab1	1	SSR	FORWARD	607	20	55.91	40.00	2.00	0.00	GATTTCTTTCTTCCCCTCAA	145	925	925	6.00	3.00	TTTC	4	tttctttctttc	12
18	DB081A05_R2.ab1	1	SSR	REVERSE	751	21	55.20	47.62	4.00	0.00	GCGTACAGATGTCAGAGAAAG
19	DB080G17_R1.ab1	1	SSR	FORWARD	724	21	54.89	42.86	4.00	3.00	AACCAGTTCAGTTCACTCTGA	162	921	921	5.00	3.00	TAAA	4	taaataaataaa	12
20	DB080G17_R1.ab1	1	SSR	REVERSE	885	21	55.12	42.86	2.00	1.00	AGAGTTTGTGTAGCAGGTTGA
21	DB080F05_R1.ab1	1	SSR	FORWARD	663	22	55.00	40.91	3.00	0.00	GTCAGGAACATAGAAGTGGTTT	142	919	919	5.00	3.00	TCTGG	5	tctggtctggtctgg	15
22	DB080F05_R1.ab1	1	SSR	REVERSE	804	20	55.14	40.00	5.00	3.00	CCTTGAAATTGTTTCTACGC
23	DB085C05_R1.ab1	1	SSR	FORWARD	655	20	55.37	40.00	4.00	1.00	CTGATCTGAAACTTGGCATT	149	913	913	5.00	2.00	GCC	3	gccgccgccgccgcc	15
24	DB085C05_R1.ab1	1	SSR	REVERSE	803	21	54.51	42.86	4.00	1.00	TCTGGATCATGGATGTCTACT
25	DB085M03_R1.ab1	1	SSR	FORWARD	100	19	55.23	47.37	8.00	2.00	TAATGGTCACGTGAGATGG	134	913	913	6.00	2.00	GTC	3	gtcgtcgtcgtc	12
26	DB085M03_R1.ab1	1	SSR	REVERSE	233	20	55.02	40.00	4.00	3.00	TAATTTGACCTGACGACACA
27	DB088L13_F1.ab1	1	SSR	FORWARD	581	21	54.98	42.86	6.00	2.00	ATGTCTCTTAATTCCGAGGTC	145	910	910	5.00	0.00	ATT	3	attattattatt	12
28	DB088L13_F1.ab1	1	SSR	REVERSE	725	20	54.67	40.00	2.00	0.00	ACGATTGAGAAAGTGGTTGT
29	DB086J03_R1.ab1	1	SSR	FORWARD	532	21	55.44	42.86	6.00	2.00	CCATTGTCTAGTGCTAATGGA	157	910	910	5.00	0.00	TGTG	4	tgtgtgtgtgtg	12
30	DB086J03_R1.ab1	1	SSR	REVERSE	688	21	54.70	42.86	6.00	2.00	GTTGAGCTAACAAGCTGAAAG
31	DB070A03_R1.ab1	1	SSR	FORWARD	176	20	55.35	50.00	4.00	3.00	AAGCTACGGAGGAGAAACTC	165	909	909	4.00	0.00	GCG	3	gcggcggcggcg	12
32	DB070A03_R1.ab1	1	SSR	REVERSE	340	20	55.51	40.00	4.00	2.00	CAATCCTGTCTCGAAGAAAA
33	DB083J20_F1.ab1	1	SSR	FORWARD	532	18	53.85	55.56	4.00	1.00	GACCTCTTCAACGTCCAG	170	908	908	5.00	1.00	CAG	3	cagcagcagcagcag	15
34	DB083J20_F1.ab1	1	SSR	REVERSE	701	21	54.88	42.86	3.00	2.00	GTGGACAGACACATAAAAAGC
35	DB083J20_F1.ab1	1	SSR	FORWARD	163	18	55.47	50.00	8.00	3.00	TAAAAGTGGCCACGTCAC	147	908	908	3.00	0.00	CACA	4	cacacacacaca	12
36	DB083J20_F1.ab1	1	SSR	REVERSE	309	21	54.75	42.86	6.00	2.00	ATATATGCTCTGCTGATGCTC
37	DB083J20_F1.ab1	1	SSR	FORWARD	203	19	54.04	42.11	6.00	0.00	GCAAACAATGCATATCCTC	149	908	908	4.00	0.00	CAAG	4	caagcaagcaag	12
38	DB083J20_F1.ab1	1	SSR	REVERSE	351	21	56.22	42.86	4.00	0.00	GCTCGCCATTATACTTGACTT
39	DB083J20_F1.ab1	1	SSR	FORWARD	681	21	54.88	42.86	3.00	2.00	GCTTTTTATGTGTCTGTCCAC	180	908	908	3.00	2.00	CTAG	4	ctagctagctag	12
40	DB083J20_F1.ab1	1	SSR	REVERSE	860	21	54.80	42.86	4.00	2.00	GTCTGAATAGTTTGACGATGG
41	DB091N01_R1.ab1	1	SSR	FORWARD	638	21	54.82	42.86	3.00	3.00	ATGAGGAGGTTGGTAAGATTC	161	908	908	4.00	0.00	ACCA	4	accaaccaacca	12
42	DB091N01_R1.ab1	1	SSR	REVERSE	798	21	54.74	42.86	3.00	0.00	GAGGTTCTTTTTCTCCAGTTC
43	DB069A20_F1.ab1	1	SSR	FORWARD	626	20	55.16	40.00	6.00	2.00	AATATTGGACATGCGAAGAC	169	907	907	4.00	2.00	AAG	3	aagaagaagaag	12
44	DB069A20_F1.ab1	1	SSR	REVERSE	794	21	54.99	47.62	5.00	3.00	GATGCAGCCTGTGTATCTTAC
45	DB079L09_R1.ab1	1	SSR	FORWARD	477	18	55.41	50.00	4.00	1.00	ATCGAGGCCCATACTGTT	182	907	907	3.00	0.00	AAAG	4	aaagaaagaaag	12
46	DB079L09_R1.ab1	1	SSR	REVERSE	658	22	54.89	40.91	3.00	0.00	CCTTTCTTTAGGGTAGTGATTG
47	DB091I18_R1.ab1	1	SSR	FORWARD	552	21	54.92	42.86	3.00	0.00	CTTTCTTCCTCCTCAGTTTTC	153	906	906	4.00	2.00	AAT	3	aataataataat	12
48	DB091I18_R1.ab1	1	SSR	REVERSE	704	21	55.57	47.62	5.00	3.00	GTAGCTGCTTTGCTATCACTG
49	DB060H07_F1.ab1	1	SSR	FORWARD	168	21	54.49	42.86	4.00	2.00	CCCTATGATGGCTACATTAGA	149	906	906	4.00	2.00	AGGTTC	6	aggttcaggttcaggttc	18
50	DB060H07_F1.ab1	1	SSR	REVERSE	316	21	55.90	42.86	2.00	1.00	ATGCTATGAACCAAGAACCTC
51	DB070A19_R1.ab1	1	SSR	FORWARD	234	20	55.51	40.00	7.00	2.00	CGTTTGGTAACCATCATTTC	193	905	905	5.00	3.00	AAG	3	aagaagaagaag	12
52	DB070A19_R1.ab1	1	SSR	REVERSE	426	21	54.06	42.86	4.00	2.00	GCTAGATGGAGAAGAAGCATA
53	DB081A13_R2.ab1	1	SSR	FORWARD	29	19	55.00	42.11	3.00	2.00	CAACAAACAAATCCTGTGC	212	905	905	5.00	1.00	ATGCCG	6	atgccgatgccgatgccg	18
54	DB081A13_R2.ab1	1	SSR	REVERSE	240	21	55.17	42.86	2.00	0.00	ATCTTGTCTTCCGTGTATGTG
55	DH021M07_F1.ab1	1	SSR	FORWARD	25	21	55.16	42.86	5.00	3.00	GTTCATTGATAGTGCCACAGT	171	905	905	3.00	2.00	GAA	3	gaagaagaagaa	12
56	DH021M07_F1.ab1	1	SSR	REVERSE	195	21	55.33	52.38	5.00	2.00	GACCAACGGTCTAGGATACTC
57	DB070E17_R1.ab1	1	SSR	FORWARD	195	20	55.28	50.00	2.00	1.00	CTATGCTTGGCTTCTCTGTC	144	903	903	4.00	1.00	TGTG	4	tgtgtgtgtgtg	12
58	DB070E17_R1.ab1	1	SSR	REVERSE	338	21	56.26	42.86	6.00	1.00	TAAAGCTTGAAGAACCTCCAC
59	DB087A01_F1.ab1	1	SSR	FORWARD	51	20	55.33	40.00	4.00	2.00	ACGTTCCCAACTCCTTTTAT	112	903	903	3.00	0.00	TCGA	4	tcgatcgatcga	12
60	DB087A01_F1.ab1	1	SSR	REVERSE	162	20	55.14	40.00	6.00	0.00	TAATTAGGCTGCTTTGCTTC
61	DB156N22_F1.ab1	1	SSR	FORWARD	15	21	55.50	42.86	8.00	2.00	CTCGTGTTACAACAAGACGTT	123	901	901	4.00	1.00	CCAA	4	ccaaccaaccaa	12
62	DB156N22_F1.ab1	1	SSR	REVERSE	137	21	55.19	52.38	8.00	3.00	CTCTGATAGCTGTCGAGTCAC
63	DB090H12_F1.ab1	1	SSR	FORWARD	710	21	54.96	47.62	3.00	0.00	GTAGTCTCACCTTTCCCTTGT	139	901	901	4.00	3.00	CATG	4	catgcatgcatg	12
64	DB090H12_F1.ab1	1	SSR	REVERSE	848	21	54.28	42.86	4.00	1.00	CTAAGTGCCAAGATTGAAGAC
65	DB079C21_F1.ab1	1	SSR	FORWARD	84	18	55.17	55.56	4.00	1.00	ACCACTCCAGTAGCAGCA	174	899	899	4.00	2.00	CAG	3	cagcagcagcag	12
66	DB079C21_F1.ab1	1	SSR	REVERSE	257	20	55.08	40.00	8.00	0.00	TTCTTTCCATGGATGTTCTC
67	DB079C21_F1.ab1	1	SSR	FORWARD	774	21	55.86	47.62	3.00	2.00	TACACTGAACCAGACGGAGTA	123	899	899	3.00	0.00	CACA	4	cacacacacacacaca	16
68	DB079C21_F1.ab1	1	SSR	REVERSE	896	20	55.24	40.00	4.00	2.00	GTGAGCTGAAAATGAAAAGC
69	DB092L23_F1.ab1	1	SSR	FORWARD	433	20	52.42	40.00	4.00	0.00	GAACCAGCTCAATTTAACAC	141	899	899	4.00	0.00	TTTG	4	tttgtttgtttg	12
70	DB092L23_F1.ab1	1	SSR	REVERSE	573	20	55.18	40.00	4.00	2.00	TTTGTACCCCATTTGATAGC
71	DB080K19_R1.ab1	1	SSR	FORWARD	675	21	55.25	42.86	6.00	0.00	CGATTAATAGAGGAGGGAGAA	152	898	898	4.00	3.00	CTCT	4	ctctctctctct	12
72	DB080K19_R1.ab1	1	SSR	REVERSE	826	22	54.91	40.91	6.00	2.00	AGTTTGCCCAGGTATACTTCTA
73	DB091M22_R1.ab1	1	SSR	FORWARD	462	22	55.16	40.91	4.00	2.00	CATTCTGTTTGAAGACGTACAG	147	898	898	4.00	1.00	ATTA	4	attaattaatta	12
74	DB091M22_R1.ab1	1	SSR	REVERSE	608	21	55.04	42.86	4.00	1.00	CAATCTACATAGCCCAGTTTG
75	DH019O05_R1.ab1	1	SSR	FORWARD	140	20	54.55	40.00	2.00	0.00	CAAACACATTCATCCACATC	131	896	896	5.00	2.00	ACAT	4	acatacatacatacat	16
76	DH019O05_R1.ab1	1	SSR	REVERSE	270	21	55.25	47.62	6.00	3.00	GGTTAGTGTTAGACACGGTGA
77	DB094F22_F1.ab1	1	SSR	FORWARD	92	21	55.09	42.86	4.00	1.00	AAGGTGAACTCTTCCTTCAAC	149	896	896	4.00	2.00	GCT	3	gctgctgctgct	12
78	DB094F22_F1.ab1	1	SSR	REVERSE	240	21	54.33	42.86	5.00	1.00	AAGTCTTCTGGCTCAGTGTAA
79	DB091D08_F1.ab1	1	SSR	FORWARD	155	21	54.42	42.86	4.00	0.00	CACCTTTCCTTAACTCTTCCT	156	895	895	4.00	2.00	CCTTC	5	ccttcccttcccttc	15
80	DB091D08_F1.ab1	1	SSR	REVERSE	310	21	54.95	42.86	3.00	0.00	AAGATGTGACTGCCATAGAGA
81	DB090B04_F1.ab1	1	SSR	FORWARD	91	21	55.11	42.86	5.00	3.00	GATTTATAGCGTCCTGGAGAT	168	894	894	5.00	1.00	GGAA	4	ggaaggaaggaa	12
82	DB090B04_F1.ab1	1	SSR	REVERSE	258	20	54.22	45.00	4.00	2.00	CAGCACAATAAACACGTACC
83	DB078M04_R1.ab1	1	SSR	FORWARD	182	21	54.85	42.86	3.00	2.00	CTTCTTGTTCTGCCATCTCTA	148	893	893	5.00	1.00	TTC	3	ttcttcttcttcttc	15
84	DB078M04_R1.ab1	1	SSR	REVERSE	329	20	55.01	40.00	6.00	0.00	GCCTCTGTTAAGAAAAGCAA
85	DB079H19_R1.ab1	1	SSR	FORWARD	111	21	53.88	47.62	6.00	1.00	AGTGTGCCTAGGTCATGTAGT	151	892	892	5.00	3.00	GTTT	4	gtttgtttgttt	12
86	DB079H19_R1.ab1	1	SSR	REVERSE	261	21	54.78	47.62	8.00	2.00	ACTCTTAAGGGTACCATCACC
87	DH031N02_F1.ab1	1	SSR	FORWARD	307	21	55.11	42.86	4.00	2.00	GGGATTAGCTGTTGTAAAGGT	133	891	891	4.00	3.00	TGCA	4	tgcatgcatgcatgcatgca	20
88	DH031N02_F1.ab1	1	SSR	REVERSE	439	21	56.53	42.86	2.00	2.00	CAGTTTCTTCATTTCCTACGG
89	DB070O21_F1.ab1	1	SSR	FORWARD	11	21	54.92	42.86	3.00	0.00	GGAGTTGACACAAAGTGAAAG	141	891	891	3.00	0.00	TATC	4	tatctatctatc	12
90	DB070O21_F1.ab1	1	SSR	REVERSE	151	21	55.83	42.86	3.00	3.00	CAAAATAAGGGGACAGACAGT
91	DB070O21_F1.ab1	1	SSR	FORWARD	367	21	55.20	42.86	4.00	0.00	TATGAACGTAGTAGGGCTGAA	173	891	891	4.00	0.00	GATC	4	gatcgatcgatc	12
92	DB070O21_F1.ab1	1	SSR	REVERSE	539	21	55.29	47.62	6.00	3.00	GACGAACATGTACTCCTCTGA
93	DB070O21_F1.ab1	1	SSR	FORWARD	512	21	54.69	42.86	6.00	2.00	TCGATGATCAGAGGAGTACAT	170	891	891	4.00	1.00	GCAT	4	gcatgcatgcat	12
94	DB070O21_F1.ab1	1	SSR	REVERSE	681	21	55.07	42.86	2.00	2.00	GGTCTTTCTCTTTCTCTTTCG
95	DB070O21_F1.ab1	1	SSR	FORWARD	668	21	54.89	47.62	4.00	2.00	GAAAGAGAAAGACCTCTCCTG	152	891	891	6.00	1.00	GATG	4	gatggatggatggatg	16
96	DB070O21_F1.ab1	1	SSR	REVERSE	819	20	54.87	45.00	6.00	1.00	CATGATCAACCTCTTTAGCC
97	DB070O21_F1.ab1	1	SSR	FORWARD	636	20	54.41	40.00	2.00	0.00	CACTTTGGTCTTTGGTCTTT	148	891	891	4.00	0.00	GAAAGA	6	gaaagagaaagagaaaga	18
98	DB070O21_F1.ab1	1	SSR	REVERSE	783	21	55.08	52.38	3.00	2.00	ATACCAGGAGAGAGGTGCTAC
99	DB071P01_F1.ab1	1	SSR	FORWARD	53	20	55.45	50.00	6.00	1.00	GAGATCTGGAGGAATTAGCC	161	891	891	3.00	0.00	CAG	3	cagcagcagcag	12
100	DB071P01_F1.ab1	1	SSR	REVERSE	213	20	55.09	40.00	4.00	0.00	CATCGTATAGCAGCACAAAA
101	DB071P01_F1.ab1	1	SSR	FORWARD	53	20	55.45	50.00	6.00	1.00	GAGATCTGGAGGAATTAGCC	161	891	891	3.00	0.00	TGCG	4	tgcgtgcgtgcg	12
102	DB071P01_F1.ab1	1	SSR	REVERSE	213	20	55.09	40.00	4.00	0.00	CATCGTATAGCAGCACAAAA
103	DB070F12_F1.ab1	1	SSR	FORWARD	533	21	54.92	52.38	4.00	1.00	CTCGTAGATGTGGAGTACCAG	136	891	891	3.00	3.00	CATC	4	catccatccatccatc	16
104	DB070F12_F1.ab1	1	SSR	REVERSE	668	21	54.90	42.86	4.00	1.00	AGTCCTTGACCCTAATTCAAC
105	DB068C12_F1.ab1	1	SSR	FORWARD	271	20	55.25	40.00	4.00	2.00	CATGTGCAAACGTAATCAAG	158	891	891	4.00	1.00	AAAT	4	aaataaataaat	12
106	DB068C12_F1.ab1	1	SSR	REVERSE	428	20	55.05	40.00	3.00	2.00	GCAATAAGGAATCACAAAGC
107	DB068C12_F1.ab1	1	SSR	FORWARD	620	21	54.60	42.86	4.00	1.00	TACTATGAGCAGCATGTGAGA	147	891	891	5.00	2.00	AGCC	4	agccagccagcc	12
108	DB068C12_F1.ab1	1	SSR	REVERSE	766	20	54.98	45.00	7.00	2.00	CGATCCTGGATGTCATTAGT
109	DB094E07_R1.ab1	1	SSR	FORWARD	463	21	54.45	42.86	3.00	1.00	GAAATCGTAACTCTCTTGCTG	159	891	891	3.00	1.00	GCGA	4	gcgagcgagcga	12
110	DB094E07_R1.ab1	1	SSR	REVERSE	621	20	54.89	45.00	6.00	0.00	CGGTCAGATCTAATGGAAAG
111	DB096K20_R1.ab1	1	SSR	FORWARD	1	18	55.53	55.56	6.00	1.00	CTCTGAGGATCCCTTTGG	234	891	891	4.00	2.00	AGG	3	aggaggaggaggagg	15
112	DB096K20_R1.ab1	1	SSR	REVERSE	234	19	55.35	47.37	4.00	3.00	GAATCCAGACCGTTCATTC
113	DB083D05_R1.ab1	1	SSR	FORWARD	9	18	59.22	55.56	2.00	2.00	ATGCGTAGTGGCGGAGAT	156	890	890	4.00	3.00	GCA	3	gcagcagcagca	12
114	DB083D05_R1.ab1	1	SSR	REVERSE	164	20	55.07	40.00	6.00	1.00	CAATTAGGCCAAGATTTCAG
115	DH045O04_R1.ab1	1	SSR	FORWARD	325	20	54.79	40.00	4.00	1.00	GCGCTAGATCATTGTTTTCT	156	890	890	4.00	0.00	AAAT	4	aaataaataaat	12
116	DH045O04_R1.ab1	1	SSR	REVERSE	480	21	55.92	42.86	4.00	2.00	TGATATTTAGGAGGAGGATCG
117	DB091D02_R1.ab1	1	SSR	FORWARD	711	21	55.63	42.86	5.00	3.00	TTGTAGTGTAGGCCATGATGT	151	889	889	4.00	1.00	GAGC	4	gagcgagcgagc	12
118	DB091D02_R1.ab1	1	SSR	REVERSE	861	19	54.42	42.11	6.00	2.00	CAAACTATTTGCACCAAGC
119	DB079D20_R1.ab1	1	SSR	FORWARD	684	21	55.25	42.86	6.00	0.00	CGATTAATAGAGGAGGGAGAA	152	888	888	4.00	3.00	CTCT	4	ctctctctctct	12
120	DB079D20_R1.ab1	1	SSR	REVERSE	835	22	54.91	40.91	6.00	2.00	AGTTTGCCCAGGTATACTTCTA
121	DB083J16_F1.ab1	1	SSR	FORWARD	582	21	55.70	42.86	5.00	2.00	ATTTCCTCTGACTGACAGCTT	150	887	887	3.00	2.00	CATG	4	catgcatgcatgcatgcatg	20
122	DB083J16_F1.ab1	1	SSR	REVERSE	731	21	54.93	47.62	5.00	0.00	GTAGTACGTCCACGAAGATTG
123	DB088B17_F1.ab1	1	SSR	FORWARD	747	18	57.52	55.56	5.00	0.00	GGCAAGGCTTTCTCCTTC	137	887	887	6.00	3.00	CAA	3	caacaacaacaa	12
124	DB088B17_F1.ab1	1	SSR	REVERSE	883	19	55.03	52.63	4.00	2.00	GAAGAAGACGAACCAGCTC
125	DB071C21_F1.ab1	1	SSR	FORWARD	158	19	53.84	47.37	2.00	2.00	GGAGGATACAACAACAACG	196	887	887	4.00	0.00	GCA	3	gcagcagcagca	12
126	DB071C21_F1.ab1	1	SSR	REVERSE	353	19	55.47	47.37	6.00	3.00	ATGTGCGTATACCGGATCT
127	DH020O13_R1.ab1	1	SSR	FORWARD	624	21	55.26	42.86	8.00	1.00	AGATAACGTTAGCCTTTGGTC	159	886	886	4.00	1.00	CTT	3	cttcttcttcttctt	15
128	DH020O13_R1.ab1	1	SSR	REVERSE	782	21	55.64	42.86	4.00	2.00	TCTACAATGGTGCTTCTTGAG
129	DB079K19_F1.ab1	1	SSR	FORWARD	30	19	66.34	57.89	4.00	2.00	AGCCGCCTCCGTTTCATCA	150	886	886	4.00	2.00	GCC	3	gccgccgccgcc	12
130	DB079K19_F1.ab1	1	SSR	REVERSE	179	18	56.83	55.56	4.00	0.00	GTGGGCACGAAGAAGAAG
131	DB080E03_R1.ab1	1	SSR	FORWARD	392	21	54.24	42.86	4.00	3.00	TTACTTTCTTGGCTCACTAGC	171	885	885	3.00	1.00	GCCG	4	gccggccggccg	12
132	DB080E03_R1.ab1	1	SSR	REVERSE	562	20	55.09	40.00	5.00	1.00	ATTTAATGTGGGTCGTTCTG
133	DB080E03_R1.ab1	1	SSR	FORWARD	622	21	54.47	47.62	8.00	1.00	AGCGTATACGTACATGAGTCC	174	885	885	5.00	2.00	ATGC	4	atgcatgcatgc	12
134	DB080E03_R1.ab1	1	SSR	REVERSE	795	21	53.63	42.86	5.00	2.00	AGCAGATTCTCTCTTCTCGTA
135	DB085J15_R1.ab1	1	SSR	FORWARD	48	21	55.11	47.62	3.00	0.00	TCAGTCTCATCCACACTTACC	155	885	885	6.00	2.00	AGCT	4	agctagctagct	12
136	DB085J15_R1.ab1	1	SSR	REVERSE	202	21	54.73	47.62	4.00	2.00	GTGTACGCCTATGTATGGAAC
137	DB085J15_R1.ab1	1	SSR	FORWARD	713	21	55.44	52.38	6.00	2.00	ACTCCTCAGTCCTGACTCACT	170	885	885	4.00	2.00	GCAC	4	gcacgcacgcacgcac	16
138	DB085J15_R1.ab1	1	SSR	REVERSE	882	19	55.65	42.11	6.00	0.00	AATTAATGGCAAGCGAGAG
139	DB156I21_F1.ab1	1	SSR	FORWARD	384	21	55.18	47.62	4.00	2.00	CGAGATAAGTGTGGATAGCTG	161	883	883	3.00	2.00	CTG	3	ctgctgctgctg	12
140	DB156I21_F1.ab1	1	SSR	REVERSE	544	21	55.08	52.38	4.00	2.00	ACTAGACAGGGCTCAGGATAC
141	DB070B18_F1.ab1	1	SSR	FORWARD	328	19	55.78	47.37	2.00	0.00	GATTTCGCAGTTCAGTTCC	167	883	883	4.00	1.00	CACG	4	cacgcacgcacg	12
142	DB070B18_F1.ab1	1	SSR	REVERSE	494	21	55.51	52.38	6.00	3.00	CCCACTAGTTCCCTCTGATAG
143	DB087D11_R1.ab1	1	SSR	FORWARD	730	20	55.77	40.00	8.00	3.00	CCCCTTCTTTTTAAAACTCG	139	883	883	3.00	1.00	AGA	3	agaagaagaaga	12
144	DB087D11_R1.ab1	1	SSR	REVERSE	868	18	55.56	55.56	4.00	2.00	GGTCCAGTCCAATCTGGT
145	DB068C19_F1.ab1	1	SSR	FORWARD	233	21	55.11	42.86	4.00	0.00	CATGATTCTGTTCTCACACCT	176	883	883	4.00	0.00	AAAAAT	6	aaaaataaaaataaaaat	18
146	DB068C19_F1.ab1	1	SSR	REVERSE	408	19	55.28	57.89	4.00	0.00	GCTAGTGGTCTGGTCTCGT
147	DB082L20_F1.ab1	1	SSR	FORWARD	672	20	54.63	40.00	4.00	3.00	CCTAGCAAACCATTACGTTT	161	883	883	3.00	0.00	AAT	3	aataataataataat	15
148	DB082L20_F1.ab1	1	SSR	REVERSE	832	21	55.15	52.38	4.00	1.00	AGAGAGACAGGGAGATCAGAG
149	DB090B16_R1.ab1	1	SSR	FORWARD	683	21	55.37	47.62	7.00	0.00	ATCCTACAGTAGATGCGTGTG	125	883	883	5.00	2.00	TCGA	4	tcgatcgatcga	12
150	DB090B16_R1.ab1	1	SSR	REVERSE	807	21	55.06	42.86	4.00	2.00	GAAAGATCGCTGTACTTGCTA
151	DB088N02_R1.ab1	1	SSR	FORWARD	629	20	55.20	40.00	5.00	0.00	AAATCTGAGACGTGGAAATG	156	882	882	4.00	1.00	AGCT	4	agctagctagct	12
152	DB088N02_R1.ab1	1	SSR	REVERSE	784	21	55.19	42.86	5.00	2.00	GTAAGCTCGGAGGATAAATGT
153	DB070G22_R1.ab1	1	SSR	FORWARD	40	21	56.01	42.86	5.00	3.00	AATCCCGCTTATGTCAGATAG	139	882	882	5.00	3.00	CATG	4	catgcatgcatg	12
154	DB070G22_R1.ab1	1	SSR	REVERSE	178	21	56.92	42.86	6.00	3.00	GCCAGCTTCATGATACAAGAT
155	DB091J10_R1.ab1	1	SSR	FORWARD	558	20	55.46	40.00	5.00	3.00	CGCTAGAATTTGAAGTTTCG	162	882	882	4.00	0.00	TGCA	4	tgcatgcatgca	12
156	DB091J10_R1.ab1	1	SSR	REVERSE	719	21	53.79	42.86	4.00	0.00	ACCTACGGATAGCTGTTAATG
157	DH019C03_R1.ab1	1	SSR	FORWARD	599	22	55.21	40.91	6.00	1.00	CTGACATATGGTTTGACCTACA	179	882	882	4.00	1.00	CTCT	4	ctctctctctct	12
158	DH019C03_R1.ab1	1	SSR	REVERSE	777	19	55.46	52.63	8.00	2.00	GCCGTACGGTAGTTAGCAT
159	DB070B14_F1.ab1	1	SSR	FORWARD	33	21	54.57	42.86	3.00	0.00	GAACCACTTATCTTTCCTTCC	159	882	882	2.00	0.00	TTTAT	5	tttattttattttat	15
160	DB070B14_F1.ab1	1	SSR	REVERSE	191	21	55.77	47.62	3.00	2.00	TATCTACCGACGCTCCTCTAT
161	DB080I06_F1.ab1	1	SSR	FORWARD	71	21	55.45	42.86	8.00	2.00	AGCAGCACTAGTGTCAAGAAA	166	881	881	4.00	3.00	AAT	3	aataataataataat	15
162	DB080I06_F1.ab1	1	SSR	REVERSE	236	21	55.72	42.86	4.00	3.00	TTTGTGGTCTACTTTGAGCAG
163	DB070M21_F1.ab1	1	SSR	FORWARD	245	20	54.44	40.00	4.00	2.00	GAAACGAGAGGGTGAAATTA	152	881	881	5.00	1.00	CGG	3	cggcggcggcgg	12
164	DB070M21_F1.ab1	1	SSR	REVERSE	396	19	54.92	52.63	4.00	0.00	CACTTGCTAGGGGTTTCTC
165	DB070M21_F1.ab1	1	SSR	FORWARD	245	20	54.44	40.00	4.00	2.00	GAAACGAGAGGGTGAAATTA	152	881	881	5.00	1.00	GGC	3	ggcggcggcggc	12
166	DB070M21_F1.ab1	1	SSR	REVERSE	396	19	54.92	52.63	4.00	0.00	CACTTGCTAGGGGTTTCTC
167	DB156C03_F1.ab1	1	SSR	FORWARD	614	21	55.87	42.86	3.00	2.00	GTGGGTGACTTTTTCAGTTCT	148	880	880	3.00	2.00	GTAC	4	gtacgtacgtac	12
168	DB156C03_F1.ab1	1	SSR	REVERSE	761	21	55.96	52.38	8.00	1.00	ACTCCCGTACGGTACTACAAG
169	DB085E22_R1.ab1	1	SSR	FORWARD	146	19	54.85	52.63	4.00	2.00	AATGGAGGACGTAGTGGTC	166	880	880	5.00	3.00	CCGTCG	6	ccgtcgccgtcgccgtcg	18
170	DB085E22_R1.ab1	1	SSR	REVERSE	311	21	54.71	42.86	4.00	1.00	CACTAGAACGATGAGAGCATT
171	DB091D23_R1.ab1	1	SSR	FORWARD	384	21	54.94	47.62	4.00	2.00	GTACTTGGTGACTTGCTTCAC	156	880	880	7.00	2.00	CAA	3	caacaacaacaa	12
172	DB091D23_R1.ab1	1	SSR	REVERSE	539	21	55.04	42.86	7.00	1.00	CTACTTTCACAAAGTGCTGCT
173	DB091D23_R1.ab1	1	SSR	FORWARD	384	21	54.94	47.62	4.00	2.00	GTACTTGGTGACTTGCTTCAC	156	880	880	7.00	2.00	ATAT	4	atatatatatat	12
174	DB091D23_R1.ab1	1	SSR	REVERSE	539	21	55.04	42.86	7.00	1.00	CTACTTTCACAAAGTGCTGCT
175	DB086K17_F1.ab1	1	SSR	FORWARD	125	20	55.14	50.00	4.00	0.00	ACGTCTCTCTTTTCCTCTCC	148	880	880	3.00	2.00	ACAC	4	acacacacacac	12
176	DB086K17_F1.ab1	1	SSR	REVERSE	272	21	55.63	42.86	7.00	2.00	TATGCCATACTAAGGTTGCAG
177	DB086K17_F1.ab1	1	SSR	FORWARD	538	21	54.80	42.86	7.00	3.00	CGGAATTGTCACTATTGAGTC	153	880	880	4.00	3.00	TGTG	4	tgtgtgtgtgtg	12
178	DB086K17_F1.ab1	1	SSR	REVERSE	690	21	55.26	42.86	2.00	1.00	TAGAGATGAGCCAAGAACTGA
179	DB079L07_R1.ab1	1	SSR	FORWARD	4	21	55.06	47.62	8.00	1.00	ACTCTGAGGATCCTTTACCTG	141	880	880	3.00	2.00	GAGA	4	gagagagagagagagagaga	20
180	DB079L07_R1.ab1	1	SSR	REVERSE	144	21	55.26	42.86	6.00	1.00	GTCTCGTTCCGTATACATTCA
181	DB070A17_R1.ab1	1	SSR	FORWARD	509	21	55.12	42.86	5.00	0.00	TTACACTCAGAGCACCTGTTT	141	878	878	5.00	3.00	CTT	3	cttcttcttctt	12
182	DB070A17_R1.ab1	1	SSR	REVERSE	649	20	53.59	40.00	4.00	2.00	TATTAAGTTGCCTCACGGTA
183	DB088L23_F1.ab1	1	SSR	FORWARD	436	21	54.78	42.86	4.00	2.00	GGCCAGTTTAGGTTAATTCTC	165	878	878	3.00	1.00	GTAC	4	gtacgtacgtac	12
184	DB088L23_F1.ab1	1	SSR	REVERSE	600	21	55.12	42.86	8.00	0.00	TGCTCCGTATAGTAGCAAAAG
185	DB088N23_F1.ab1	1	SSR	FORWARD	629	20	59.38	60.00	4.00	0.00	GTGGCCTACGACTGCTACCT	164	878	878	5.00	2.00	CTC	3	ctcctcctcctc	12
186	DB088N23_F1.ab1	1	SSR	REVERSE	792	20	55.14	40.00	2.00	2.00	ACAAAACACACGCAGACATA
187	DB079O01_R1.ab1	1	SSR	FORWARD	619	19	55.56	47.37	3.00	0.00	AGATTGCTTCTGTGGTGGT	185	877	877	6.00	3.00	AAG	3	aagaagaagaag	12
188	DB079O01_R1.ab1	1	SSR	REVERSE	803	18	56.49	50.00	4.00	2.00	CAAGCAGTTGAAGCATCG
189	DH017E04_F1.ab1	1	SSR	FORWARD	387	21	54.86	47.62	3.00	1.00	AAGAGTGCGTAGAGTTCACAG	142	877	877	4.00	2.00	AGA	3	agaagaagaaga	12
190	DH017E04_F1.ab1	1	SSR	REVERSE	528	21	55.66	42.86	4.00	2.00	CTTCTTCTTCGATCAGCTTCT
191	DH017E04_F1.ab1	1	SSR	FORWARD	423	21	54.29	42.86	3.00	2.00	GAAGAAGAAACAGAAGTCACG	146	877	877	3.00	1.00	GAA	3	gaagaagaagaa	12
192	DH017E04_F1.ab1	1	SSR	REVERSE	568	19	54.90	57.89	8.00	1.00	CTCCCTGCAGGAGTAAGAC
193	DB085A19_R1.ab1	1	SSR	FORWARD	104	20	55.19	45.00	2.00	1.00	ACAAAAGACACTCCCAACAG	139	877	877	4.00	2.00	AACA	4	aacaaacaaaca	12
194	DB085A19_R1.ab1	1	SSR	REVERSE	242	21	55.34	47.62	6.00	2.00	GGAGACATCTCATGTATGGTG
195	DB085A19_R1.ab1	1	SSR	FORWARD	623	21	54.86	42.86	4.00	2.00	CCACTACAACGTCGATAATTC	156	877	877	5.00	0.00	AACA	4	aacaaacaaaca	12
196	DB085A19_R1.ab1	1	SSR	REVERSE	778	20	54.21	40.00	6.00	0.00	ATCCCATGTATGGTGTTTCT
197	DB080M17_R1.ab1	1	SSR	FORWARD	196	21	54.72	42.86	4.00	0.00	GAAAGTATGATGAGGTCGATG	157	877	877	4.00	0.00	AGAAA	5	agaaaagaaaagaaa	15
198	DB080M17_R1.ab1	1	SSR	REVERSE	352	21	54.95	47.62	3.00	0.00	GAGGAACTGCTGATAGTGATG
199	DB088P05_F1.ab1	1	SSR	FORWARD	229	20	55.16	40.00	7.00	1.00	TGGTTGAATGTTACATGGTG	148	877	877	3.00	0.00	AGGT	4	aggtaggtaggt	12
200	DB088P05_F1.ab1	1	SSR	REVERSE	376	21	54.79	42.86	4.00	2.00	GAGCAGAAAGAGGCTAATCTT
201	DH005D21_R1.ab1	1	SSR	FORWARD	395	21	54.71	47.62	4.00	3.00	CATCAGGAGAAGAGATGTCAC	136	877	877	4.00	3.00	CTAG	4	ctagctagctag	12
202	DH005D21_R1.ab1	1	SSR	REVERSE	530	21	54.25	42.86	4.00	0.00	TAACTTGTAAGGCACCACTCT
203	DB070O08_R1.ab1	1	SSR	FORWARD	618	19	54.76	47.37	4.00	3.00	GTTATCGAAGGAGGCATTC	170	877	877	5.00	0.00	GCG	3	gcggcggcggcg	12
204	DB070O08_R1.ab1	1	SSR	REVERSE	787	20	55.37	45.00	4.00	0.00	TCTATGGAATGTACGGGTGT
205	DB070O08_R1.ab1	1	SSR	FORWARD	96	21	54.45	42.86	4.00	2.00	CTTAGATAGCATTTCCTGTCG	152	877	877	6.00	2.00	ATTA	4	attaattaatta	12
206	DB070O08_R1.ab1	1	SSR	REVERSE	247	21	55.02	47.62	4.00	0.00	CGTATCTACCAGAGAACGATG
207	DB088I01_F1.ab1	1	SSR	FORWARD	22	20	55.07	40.00	6.00	2.00	GCTAAATGCATGTGTTGCTA	146	876	876	3.00	2.00	ATT	3	attattattatt	12
208	DB088I01_F1.ab1	1	SSR	REVERSE	167	20	54.71	40.00	4.00	1.00	ATCCCGATCTTTTGACTACA
209	DB070E19_R1.ab1	1	SSR	FORWARD	54	19	55.31	47.37	2.00	0.00	TCCTCTCGGTTGTCTTTTC	228	876	876	6.00	3.00	CTCT	4	ctctctctctct	12
210	DB070E19_R1.ab1	1	SSR	REVERSE	281	21	54.73	42.86	2.00	2.00	ACAGATTAGCAAGAGGGAGAT
211	DB070E19_R1.ab1	1	SSR	FORWARD	362	20	55.46	50.00	4.00	2.00	GGAGATCAAGTCATGGAGTG	172	876	876	4.00	1.00	TTTTTA	6	tttttatttttattttta	18
212	DB070E19_R1.ab1	1	SSR	REVERSE	533	21	55.07	47.62	7.00	2.00	CACCTGGTGTTAGTGATGAGT
213	DB092B07_R1.ab1	1	SSR	FORWARD	588	21	54.26	42.86	4.00	2.00	GCTATGGAGTTTCATTACAGC	200	876	876	4.00	1.00	TCT	3	tcttcttcttct	12
214	DB092B07_R1.ab1	1	SSR	REVERSE	787	18	55.17	55.56	6.00	3.00	TCCGACACGCACTAGTCT
215	DB083N20_F1.ab1	1	SSR	FORWARD	675	21	54.96	42.86	2.00	2.00	GGTAAACAAGAGAAGGAAAGC	155	875	875	5.00	0.00	TTC	3	ttcttcttcttc	12
216	DB083N20_F1.ab1	1	SSR	REVERSE	829	20	54.69	40.00	5.00	2.00	CCTGCTTTAAGGGATGTAAA
217	DB092B01_R1.ab1	1	SSR	FORWARD	654	18	54.83	55.56	4.00	3.00	GAAGCCGTGCCTGTACTA	149	875	875	4.00	2.00	AGC	3	agcagcagcagcagc	15
218	DB092B01_R1.ab1	1	SSR	REVERSE	802	21	55.70	42.86	3.00	1.00	TCTTGTAACGGAGAAGGAAGT
219	DB085N06_R1.ab1	1	SSR	FORWARD	632	18	55.41	55.56	4.00	3.00	GAGCTTGAGCCTGATCCT	160	875	875	5.00	1.00	TCG	3	tcgtcgtcgtcgtcg	15
220	DB085N06_R1.ab1	1	SSR	REVERSE	791	19	55.30	52.63	5.00	2.00	TCCCTCAGGATGTACCTGT
221	DB083E03_R1.ab1	1	SSR	FORWARD	337	21	55.71	42.86	6.00	2.00	TCTTAAGTTCACGTTCAGTGC	165	875	875	4.00	1.00	TTTC	4	tttctttctttc	12
222	DB083E03_R1.ab1	1	SSR	REVERSE	501	21	54.90	42.86	4.00	2.00	GTCCCATTTTAGTCTTGACCT
223	DB080B01_R1.ab1	1	SSR	FORWARD	595	22	55.83	40.91	4.00	0.00	GCTCAAGATTCAAGAACAGAAC	150	874	874	5.00	3.00	GCA	3	gcagcagcagcagca	15
224	DB080B01_R1.ab1	1	SSR	REVERSE	744	20	54.86	45.00	4.00	0.00	GTTTCCATGTCGCTATCTTC
225	DB079D24_R1.ab1	1	SSR	FORWARD	291	21	55.68	42.86	4.00	2.00	GAATGAAAGAGCGAAGCTAGT	175	874	874	4.00	1.00	CATG	4	catgcatgcatg	12
226	DB079D24_R1.ab1	1	SSR	REVERSE	465	20	54.98	40.00	3.00	0.00	TGTGTGCTGTCAAAAAGAAG
227	DB079D24_R1.ab1	1	SSR	FORWARD	291	21	55.68	42.86	4.00	2.00	GAATGAAAGAGCGAAGCTAGT	175	874	874	4.00	1.00	GCTT	4	gcttgcttgctt	12
228	DB079D24_R1.ab1	1	SSR	REVERSE	465	20	54.98	40.00	3.00	0.00	TGTGTGCTGTCAAAAAGAAG
229	DB079D24_R1.ab1	1	SSR	FORWARD	446	20	54.98	40.00	3.00	1.00	CTTCTTTTTGACAGCACACA	140	874	874	3.00	0.00	TTGC	4	ttgcttgcttgc	12
230	DB079D24_R1.ab1	1	SSR	REVERSE	585	21	54.96	42.86	5.00	2.00	ATTACTACCATGATGCACACC
231	DB079D24_R1.ab1	1	SSR	FORWARD	446	20	54.98	40.00	3.00	1.00	CTTCTTTTTGACAGCACACA	140	874	874	3.00	0.00	TGCA	4	tgcatgcatgca	12
232	DB079D24_R1.ab1	1	SSR	REVERSE	585	21	54.96	42.86	5.00	2.00	ATTACTACCATGATGCACACC
233	DB070C17_F1.ab1	1	SSR	FORWARD	356	21	55.15	42.86	6.00	2.00	CGTCTTTTGTTCTAACACCTG	184	874	874	3.00	0.00	TGCT	4	tgcttgcttgct	12
234	DB070C17_F1.ab1	1	SSR	REVERSE	539	21	55.10	52.38	7.00	2.00	CTACTAGCTAAGCTGGGCTCT
235	DB092F21_R1.ab1	1	SSR	FORWARD	283	21	55.85	52.38	4.00	3.00	GATCAGTACGTGTCGTCTCTG	167	874	874	4.00	0.00	CCGG	4	ccggccggccgg	12
236	DB092F21_R1.ab1	1	SSR	REVERSE	449	21	55.07	42.86	4.00	0.00	CCATCTGGTCTCTTTACCTTT
237	DB086L22_R1.ab1	1	SSR	FORWARD	404	21	55.85	42.86	7.00	3.00	GCATCGACTTGATGTGATAAG	139	874	874	4.00	2.00	GAT	3	gatgatgatgat	12
238	DB086L22_R1.ab1	1	SSR	REVERSE	542	20	54.47	40.00	2.00	0.00	GCCAACAACTAAACAAACCT
239	DB079M19_R1.ab1	1	SSR	FORWARD	205	21	55.17	42.86	6.00	2.00	CATCATAAAGTGTCGACTGGT	169	874	874	3.00	2.00	GCTA	4	gctagctagcta	12
240	DB079M19_R1.ab1	1	SSR	REVERSE	373	20	55.19	50.00	6.00	2.00	TAGGACTAGCGGTAGCATGT
241	DB069A15_R1.ab1	1	SSR	FORWARD	337	21	54.88	42.86	7.00	1.00	GATCCTGCTGTAACAACAAAC	154	873	873	6.00	1.00	GCA	3	gcagcagcagca	12
242	DB069A15_R1.ab1	1	SSR	REVERSE	490	21	55.79	42.86	5.00	1.00	AACTTCCACCAAGATTCTGTC
243	DB081F15_F1.ab1	1	SSR	FORWARD	531	21	54.67	42.86	7.00	2.00	TCAAGATTTAGTCTGGAGTGC	165	873	873	3.00	1.00	TTTGC	5	tttgctttgctttgc	15
244	DB081F15_F1.ab1	1	SSR	REVERSE	695	21	54.96	42.86	4.00	1.00	AAGAGCTTATTGTCTGTGCTG
245	DB087O04_F1.ab1	1	SSR	FORWARD	240	21	54.50	42.86	4.00	2.00	ATATTCCACACCAGAGGTACA	134	873	873	5.00	2.00	AAATG	5	aaatgaaatgaaatg	15
246	DB087O04_F1.ab1	1	SSR	REVERSE	373	21	56.12	42.86	5.00	1.00	CGGTTCCGGTATATTATTGTC
247	DB092D09_R1.ab1	1	SSR	FORWARD	46	21	54.81	42.86	5.00	0.00	CTGAAGTTAAACACCCACAAC	162	873	873	4.00	0.00	GCAT	4	gcatgcatgcatgcat	16
248	DB092D09_R1.ab1	1	SSR	REVERSE	207	20	55.81	45.00	3.00	0.00	CATCCAGTCAGAAGCAAATC
249	DB070I17_R1.ab1	1	SSR	FORWARD	402	21	54.77	42.86	4.00	0.00	TCTCTACGTTTCTTGGACTTG	147	872	872	4.00	2.00	AAC	3	aacaacaacaac	12
250	DB070I17_R1.ab1	1	SSR	REVERSE	548	21	54.93	52.38	6.00	2.00	AGGAGTGAGTACCCTCCATAG
251	DB091P10_R1.ab1	1	SSR	FORWARD	136	21	55.43	42.86	2.00	0.00	TTATCTCTCTTGCTCCTTTCC	139	872	872	3.00	0.00	CTCT	4	ctctctctctctctct	16
252	DB091P10_R1.ab1	1	SSR	REVERSE	274	20	55.46	40.00	6.00	2.00	TCACCGAATTAAATCTGCTC
253	DH019O21_R1.ab1	1	SSR	FORWARD	587	20	54.89	50.00	4.00	0.00	AGATCCTTCTCCTCTCCAAC	158	872	872	3.00	1.00	ATGA	4	atgaatgaatga	12
254	DH019O21_R1.ab1	1	SSR	REVERSE	744	21	54.89	47.62	4.00	2.00	ACTGGCTGCGTGTACTTATAG
255	DH021L21_R1.ab1	1	SSR	FORWARD	694	21	54.95	42.86	4.00	1.00	TGTTGTTCGACCTCAGTTAGT	138	872	872	3.00	0.00	TCC	3	tcctcctcctcc	12
256	DH021L21_R1.ab1	1	SSR	REVERSE	831	21	54.92	42.86	4.00	2.00	AAGGATCTCCAGACTTCATTC
257	DB078A15_R1.ab1	1	SSR	FORWARD	633	21	55.93	47.62	4.00	2.00	GATAGTCATGTCGTTGCAGAG	152	872	872	6.00	3.00	AGA	3	agaagaagaagaagaagaaga	21
258	DB078A15_R1.ab1	1	SSR	REVERSE	784	22	54.98	50.00	6.00	2.00	GAGATCGAGCTAACTACAGACC
259	DB078A15_R1.ab1	1	SSR	FORWARD	702	20	54.22	40.00	6.00	2.00	GGCCAGAGAAATTAATAACG	154	872	872	3.00	1.00	CTG	3	ctgctgctgctg	12
260	DB078A15_R1.ab1	1	SSR	REVERSE	855	20	56.27	45.00	8.00	2.00	TAACCAAGCTAGCTGCACAT
261	DB078A15_R1.ab1	1	SSR	FORWARD	54	21	55.07	47.62	8.00	2.00	ATGTCGACAGGCTACAGACTA	124	872	872	5.00	1.00	GTAT	4	gtatgtatgtat	12
262	DB078A15_R1.ab1	1	SSR	REVERSE	177	21	55.34	47.62	4.00	0.00	AGCTTGTTGGTTAGAGTAGGG
263	DB078A15_R1.ab1	1	SSR	FORWARD	702	20	54.22	40.00	6.00	2.00	GGCCAGAGAAATTAATAACG	154	872	872	3.00	1.00	CTTTCT	6	ctttctctttctctttctctttct	24
264	DB078A15_R1.ab1	1	SSR	REVERSE	855	20	56.27	45.00	8.00	2.00	TAACCAAGCTAGCTGCACAT
265	DB090B06_R1.ab1	1	SSR	FORWARD	284	21	55.08	42.86	4.00	3.00	AACTAACCATGTCCACTGTTG	169	872	872	3.00	1.00	GCAT	4	gcatgcatgcatgcat	16
266	DB090B06_R1.ab1	1	SSR	REVERSE	452	20	55.05	45.00	5.00	2.00	TAGAGGGTCTCTGCCAATTA
267	DB070K23_R1.ab1	1	SSR	FORWARD	102	20	54.75	40.00	4.00	0.00	CCAAGATTAACAGGGAAATG	155	871	871	5.00	2.00	AAAG	4	aaagaaagaaag	12
268	DB070K23_R1.ab1	1	SSR	REVERSE	256	20	56.01	45.00	4.00	2.00	CTATCGCTTTCCTTCATGTG
269	DH046C21_F1.ab1	1	SSR	FORWARD	571	21	54.47	52.38	5.00	1.00	GTACGTCCTACCTTAGCTCCT	151	870	870	4.00	0.00	ATCC	4	atccatccatcc	12
270	DH046C21_F1.ab1	1	SSR	REVERSE	721	20	54.79	40.00	5.00	2.00	TAAGCCTGGATCTGATTGAT
271	DB070K19_R1.ab1	1	SSR	FORWARD	67	18	55.15	50.00	4.00	0.00	ATTTCTCTCGGCCTCATC	154	870	870	5.00	0.00	TCTT	4	tctttctttctt	12
272	DB070K19_R1.ab1	1	SSR	REVERSE	220	20	55.01	45.00	3.00	0.00	CGTCACAACAGTAGCAGAAA
273	DB070K19_R1.ab1	1	SSR	FORWARD	465	21	55.27	42.86	4.00	0.00	CTAGTTAATGCCCCTTGTCTT	177	870	870	5.00	0.00	CTAT	4	ctatctatctat	12
274	DB070K19_R1.ab1	1	SSR	REVERSE	641	21	55.59	47.62	4.00	3.00	CCTTTTAACCCCTCTACACTG
275	DB087O04_R1.ab1	1	SSR	FORWARD	648	21	54.62	42.86	4.00	2.00	CTACTTCCCGGCTTTAATACT	132	870	870	4.00	2.00	AATCCT	6	aatcctaatcctaatcct	18
276	DB087O04_R1.ab1	1	SSR	REVERSE	779	21	55.27	47.62	2.00	0.00	CAGATAGGGAGAGAAAGAGGA
277	DB079B14_R1.ab1	1	SSR	FORWARD	376	21	55.13	42.86	5.00	0.00	GTGGATAAAGACAACTCCACA	195	870	870	5.00	1.00	AAAT	4	aaataaataaat	12
278	DB079B14_R1.ab1	1	SSR	REVERSE	570	21	55.01	47.62	5.00	3.00	CACAGTGGGCTGTAAAGTAAC
279	DH042D04_F1.ab1	1	SSR	FORWARD	379	21	54.89	47.62	3.00	0.00	AGAGATAGGGGAGATGATGAG	166	870	870	2.00	1.00	GAT	3	gatgatgatgat	12
280	DH042D04_F1.ab1	1	SSR	REVERSE	544	21	55.07	47.62	3.00	2.00	AAGAGAGAACTGGGTGAAGTC
281	DH031I21_R1.ab1	1	SSR	FORWARD	501	21	54.06	47.62	6.00	2.00	CGGTCGACAGATATAGAACTC	203	869	869	5.00	2.00	GAAAA	5	gaaaagaaaagaaaa	15
282	DH031I21_R1.ab1	1	SSR	REVERSE	703	20	55.66	40.00	5.00	2.00	CGAACGGTTACGTTTTTCTA
283	DB092D23_R1.ab1	1	SSR	FORWARD	91	20	54.94	50.00	3.00	2.00	CAACCCTAACCCTAAGTCCT	217	869	869	8.00	3.00	CCG	3	ccgccgccgccg	12
284	DB092D23_R1.ab1	1	SSR	REVERSE	307	21	55.96	47.62	5.00	2.00	GTCTTCGGAGTCAGAGTTGAT
285	DB091A06_R1.ab1	1	SSR	FORWARD	264	21	55.92	42.86	5.00	2.00	GCTAAAACATGATGACACGAG	155	869	869	5.00	3.00	TCTC	4	tctctctctctctctctctc	20
286	DB091A06_R1.ab1	1	SSR	REVERSE	418	20	54.84	40.00	5.00	3.00	AGCTCACAGGATTTTTGTGT
287	DH040O09_F1.ab1	1	SSR	FORWARD	38	21	56.05	42.86	4.00	2.00	TTTATGGGTTTCACCTCTAGC	155	869	869	3.00	3.00	TTG	3	ttgttgttgttgttg	15
288	DH040O09_F1.ab1	1	SSR	REVERSE	192	21	55.45	47.62	6.00	2.00	CAGTATTGGACACTACGTGCT
289	DB091M04_R1.ab1	1	SSR	FORWARD	287	21	55.11	47.62	4.00	2.00	TGTCTCTGTCCACTTGGTATC	156	869	869	5.00	3.00	ATAC	4	atacatacatac	12
290	DB091M04_R1.ab1	1	SSR	REVERSE	442	21	55.97	42.86	3.00	1.00	GGATTCTTTTGTAGCCAGTGT
291	DB081J15_F1.ab1	1	SSR	FORWARD	32	18	58.16	55.56	2.00	0.00	ATCCGTCAACTGCCTCCT	199	868	868	6.00	3.00	GCG	3	gcggcggcggcg	12
292	DB081J15_F1.ab1	1	SSR	REVERSE	230	21	55.83	47.62	4.00	3.00	GAAAGAGGAGTTCTTGTCGAG
293	DB073K12_F1.ab1	1	SSR	FORWARD	162	21	55.48	42.86	6.00	2.00	CAGGTGGTGTACATTAATTGG	149	868	868	3.00	2.00	CGG	3	cggcggcggcggcgg	15
294	DB073K12_F1.ab1	1	SSR	REVERSE	310	19	54.87	47.37	6.00	2.00	ATCCTCGTCGACATATTCC
295	DB083F22_R1.ab1	1	SSR	FORWARD	431	20	55.18	45.00	6.00	2.00	TACAATCCTAGGTGGAATCG	206	868	868	4.00	1.00	CGAT	4	cgatcgatcgat	12
296	DB083F22_R1.ab1	1	SSR	REVERSE	636	18	54.93	44.44	3.00	1.00	TTTCTTGGACGGAGATGA
297	DB088B03_F1.ab1	1	SSR	FORWARD	635	20	54.86	40.00	6.00	2.00	GCATGGATTAATTTGCTACC	158	868	868	5.00	1.00	TTAA	4	ttaattaattaa	12
298	DB088B03_F1.ab1	1	SSR	REVERSE	792	21	55.73	47.62	6.00	2.00	CTCCATCGATCAGTACATACG
299	DB068K20_F1.ab1	1	SSR	FORWARD	395	21	55.28	42.86	4.00	1.00	GTCCAAACCTATCCCTATTTG	154	868	868	4.00	3.00	CAAA	4	caaacaaacaaa	12
300	DB068K20_F1.ab1	1	SSR	REVERSE	548	21	54.84	42.86	4.00	3.00	CTACTTGGTAAGCTGGTTCAA
301	DB070O17_F1.ab1	1	SSR	FORWARD	58	21	54.16	42.86	4.00	3.00	AAAGGGACCTAAGACATTCTC	149	868	868	4.00	2.00	TCTC	4	tctctctctctctctc	16
302	DB070O17_F1.ab1	1	SSR	REVERSE	206	21	55.66	42.86	5.00	0.00	GTGGCACCTAAAAAGGAATAG
303	DB070O17_F1.ab1	1	SSR	FORWARD	648	21	54.56	42.86	6.00	1.00	TCGCATACTAGTCAAATACCC	150	868	868	4.00	1.00	AGCT	4	agctagctagct	12
304	DB070O17_F1.ab1	1	SSR	REVERSE	797	21	54.65	47.62	4.00	1.00	GCAGTAGATAACAGGTGCAGT
305	DB091J11_R1.ab1	1	SSR	FORWARD	26	19	55.54	42.11	5.00	3.00	GATCCGATGGTTTTCAAAG	180	868	868	3.00	2.00	GGA	3	ggaggaggagga	12
306	DB091J11_R1.ab1	1	SSR	REVERSE	205	20	58.12	50.00	7.00	0.00	ATATTCGAGCTGCTCCTCCT
307	DB068M21_F1.ab1	1	SSR	FORWARD	630	21	55.20	42.86	4.00	3.00	CATCAGTCAACCTCTGTTGTT	131	867	867	5.00	0.00	GATC	4	gatcgatcgatc	12
308	DB068M21_F1.ab1	1	SSR	REVERSE	760	22	55.15	40.91	4.00	1.00	GTGCTGTGAATTACTTTGAGTG
309	DB087I02_R1.ab1	1	SSR	FORWARD	675	21	55.91	42.86	2.00	0.00	CAAGAGCAATAGAGCATACGA	126	867	867	4.00	1.00	GTGCA	5	gtgcagtgcagtgca	15
310	DB087I02_R1.ab1	1	SSR	REVERSE	800	20	57.18	50.00	8.00	2.00	GTATATACGGGCACCACCAT
311	DH006M17_F1.ab1	1	SSR	FORWARD	532	20	55.34	40.00	4.00	0.00	TCGAATAGATAACGCACACA	146	867	867	3.00	1.00	CGTA	4	cgtacgtacgtacgta	16
312	DH006M17_F1.ab1	1	SSR	REVERSE	677	21	55.91	47.62	6.00	1.00	ATAATCAGTCAGCTGGTCTCC
313	DB070G19_R1.ab1	1	SSR	FORWARD	290	21	55.55	47.62	4.00	0.00	CGTACCGAGATACATCACATC	113	866	866	3.00	0.00	ATAT	4	atatatatatat	12
314	DB070G19_R1.ab1	1	SSR	REVERSE	402	21	56.07	42.86	6.00	2.00	GGCTGCAACCTCATACTTATT
315	DB070G19_R1.ab1	1	SSR	FORWARD	484	21	54.28	42.86	4.00	2.00	CAGCGTTTCTATATCCATAGG	128	866	866	4.00	2.00	GCTA	4	gctagctagcta	12
316	DB070G19_R1.ab1	1	SSR	REVERSE	611	20	55.41	50.00	6.00	0.00	CTCTTCTACGTACCGCTTTG
317	DB083B06_F1.ab1	1	SSR	FORWARD	349	18	57.32	55.56	6.00	0.00	ATCCCCTTGAGGATGGAG	283	866	866	5.00	0.00	TGC	3	tgctgctgctgc	12
318	DB083B06_F1.ab1	1	SSR	REVERSE	631	19	55.91	47.37	4.00	2.00	GCATAAGGAGGAATTGCAG
319	DH019C01_R1.ab1	1	SSR	FORWARD	639	19	55.18	42.11	2.00	0.00	ACCCAAGATGAAGCAAAAC	125	866	866	4.00	1.00	CTA	3	ctactactactacta	15
320	DH019C01_R1.ab1	1	SSR	REVERSE	763	21	54.48	42.86	4.00	2.00	ATCGAGGACCCTTGTACTAAT
321	DH035D04_R1.ab1	1	SSR	FORWARD	426	22	55.01	40.91	5.00	2.00	AGTTCTGGTCCTAGATTAAGCA	161	866	866	6.00	2.00	GCCT	4	gcctgcctgcct	12
322	DH035D04_R1.ab1	1	SSR	REVERSE	586	21	55.07	42.86	5.00	1.00	CCTTGAGAATCTAACCCAAGT
323	DB088J20_R1.ab1	1	SSR	FORWARD	330	21	55.23	42.86	5.00	2.00	TCGCCTAGTCTCAGATGATAA	131	866	866	4.00	2.00	AAAG	4	aaagaaagaaagaaag	16
324	DB088J20_R1.ab1	1	SSR	REVERSE	460	21	55.01	42.86	4.00	2.00	TGAGGATGTACTCGCTGTAAT
325	DB092F02_F1.ab1	1	SSR	FORWARD	15	21	55.45	42.86	6.00	2.00	CCTATAATTCCCAGGAAACAG	178	866	866	3.00	1.00	ACGCGA	6	acgcgaacgcgaacgcga	18
326	DB092F02_F1.ab1	1	SSR	REVERSE	192	18	55.37	55.56	4.00	2.00	TACACCTCGTCCATGCTC
327	DB092C21_R1.ab1	1	SSR	FORWARD	11	19	55.18	52.63	7.00	2.00	GGATCCTGGATGCTAGAGA	165	866	866	5.00	1.00	TTC	3	ttcttcttcttc	12
328	DB092C21_R1.ab1	1	SSR	REVERSE	175	21	55.12	42.86	2.00	2.00	AGAACACAACAGCCAAGAGTA
329	DB092M05_F1.ab1	1	SSR	FORWARD	336	21	55.78	42.86	5.00	2.00	TATATGCTGCCTGTACTTTGC	154	865	865	4.00	1.00	AAATA	5	aaataaaataaaata	15
330	DB092M05_F1.ab1	1	SSR	REVERSE	489	21	54.62	42.86	3.00	2.00	GGTTCTATTCACATCCATACG
331	DB083H01_F1.ab1	1	SSR	FORWARD	289	20	54.81	45.00	4.00	3.00	CTCTTCGATGGATTTGAGTC	169	865	865	5.00	1.00	GGT	3	ggtggtggtggt	12
332	DB083H01_F1.ab1	1	SSR	REVERSE	457	22	54.71	40.91	6.00	2.00	ACAGTGAGACATTCATCACCTA
333	DB068E05_F1.ab1	1	SSR	FORWARD	527	19	59.17	57.89	6.00	2.00	TCTCCAGCTCGATCTCGTC	165	865	865	4.00	0.00	CTG	3	ctgctgctgctg	12
334	DB068E05_F1.ab1	1	SSR	REVERSE	691	18	55.01	50.00	4.00	2.00	GATACGCTGCAACAATCC
335	DB068E05_F1.ab1	1	SSR	FORWARD	234	21	54.72	42.86	2.00	0.00	CAGTCCTAAACCCCTAAAAAC	165	865	865	3.00	1.00	GTAT	4	gtatgtatgtat	12
336	DB068E05_F1.ab1	1	SSR	REVERSE	398	18	57.54	55.56	3.00	0.00	ATCAGCGAGCAGGTCAAG
337	DH038D21_R1.ab1	1	SSR	FORWARD	496	20	55.12	40.00	3.00	1.00	ATAACTTCGGCACTCTTTGA	173	864	864	4.00	2.00	ACTA	4	actaactaacta	12
338	DH038D21_R1.ab1	1	SSR	REVERSE	668	21	54.81	42.86	6.00	0.00	ACTCAGGCCTCTGATAGTTTT