| SSR Motif | Mofif Length |
|---|---|
| Dinucleotide | >= 12 nt |
| Trinucleotide | >= 12 nt |
| Tetranucleotide | >= 12 nt |
| Pentanucleotide | >= 15 nt |
| Hexanucleotide | >= 18 nt |
| Parameter | Value |
|---|---|
| Product size | 100-300, optimum: 150 |
| Primer Tm | 50-50, optimum: 55, maximum Tm difference: 20 |
| Primer GC% | 40%-60% |
| Self complementarity | <= 8 |
| 3' self complementarity | <= 3 |
| Maximum # of N's | 0 |
| Maximum polu X's | 5 |
| Salt concentration | 50 nM |
| DNA concentration | 50 nM |